1st International Conference of Soil and Agriculture:
TOWARDS SOIL SUSTAINABILITY
11–13 June 2024 • Lublin–Nałęczów, Poland

https://doi.org/10.24326/ICSA1.PP.30
Published online: 4 October 2024

Microbiological properties of Technosols developing
under alfalfa cultivation with different fertilization rates
Natalia Tatuśko-Krygier¹*, Krzysztof Otremba¹, Michał Kozłowski¹, Katarzyna Głuchowska², Agnieszka Wolna-Maruwka², Alicja Niewiadomska²

¹ Department of Soil Science, Land Reclamation and Geodesy, Poznan University of Life Sciences, Wojska Polskiego 28, 60-637, Poznan, Poland
² Department of Soil Science and Microbiology, Poznan University of Life Sciences, Wojska Polskiego 28, 60-637 Poznan, Poland
* Corresponding author:

Abstract
Open-cast lignite mining significantly degrades the environment, especially the soil. After mining, these areas have to be reclaimed. This process has the effect of developing “new soil”, which properties, could be significantly different from that naturally developed. Until now, in research on the effectiveness of reclamation treatments, much attention has been concentrated on physical and chemical properties while less on biological properties. Biological properties of soils, especially Technosols, can be one of the indicators of soil fertility and reclamation effectiveness. The aim of this study was to determine bacterial metapopulation and enzymatic activity in Technosols under alfalfa-grass cultivation in plots with varying mineral fertilization levels after 40 years of agricultural reclamation. The experiment included 3 variants of mineral fertilization: 0NPK (no fertilization), 1NPK and 2NPK. In 2019, at the beginning of June, soil samples were taken from the topsoil layer (0–20 cm) of each plot for microbiological analysis. 16S Metagenomics, as well as the enzymes catalase, dehydrogenase, acid and alkaline phosphatase and nitrogenase were determined in the samples taken. The results indicated that for each of the analysed soil samples, more than 99% were bacteria and only 0.01% were Archaea. The analysed soil were different in terms of bacterial biodiversity. The most abundant bacterial types, regardless of the fertilization used, were Actinobacteria (34.45–40.01%), Proteobacteria (34.21–37.69%) and Firmicutes (7.61–9.51%). Nitrogen fertilization contributed to decrease bacterial replication belongs to the Actinobacteria type, and an increase content of sequences belonging to Proteobacteria and Firmicutes, which indicates early colonization of disturbed ecosystems. In the case of dehydrogenase and alkaline phosphatase activities, there were no significant differences depending on the variant of mineral fertilization. The variants with fertilization (1NPK and 2NPK) were characterized by lower acid phosphatase activity than the variant without fertility used. In fertilized Technosols, especially for the 1NPK variant, compared to the soils of the 0NPK plot, the highest activity of catalase, considered as an indicator of aerobic microbial activity, nitrogenase and the value of the biological fertility index BIF were determined.

Keywords: agriculture reclamation, microbiological properties, Technosols 

How to cite
Tatuśko-Krygier N., Otremba K., Kozłowski M., Głuchowska K., Wolna-Maruwka A., Niewiadomska A., 2024. Microbiological properties of Technosols developing under alfalfa cultivation with different fertilization rates.
1st International Conference of Soil and Agriculture: Towards Soil Sustainability. https://doi.org/10.24326/ICSA1.PP.30