Biochemistry I

Biochemistry course covers 2 semester and 150 didactic hours.

Frontal lectures (30 h per semester) are in weekly intervals during 15 consecutive weeks 2 h each and cover selected topics necessary to understand metabolism of aminoacids, nucleic acids, sugars and lipids. Moreover, the control and regulation of metabolic pathways is discussed.

Practical classes (45 h per semester) are obligatory and are in weekly intervals during 15 consecutive weeks 3 h each. Students carry on chemical experiments by their own. Students should be prepared for coming class.

Detailed rules of laboratory classes and work safety are available on web page and will be described during the first class. White coat is required for practical classes.

Students get access to digital platform with virtual cases for self-study.

Detailed description of topics is available in Syllabuses.

Lectures are held in Lecture hall A – Collegium Veterinarium

Practicals are held in laboratory no 120 – Collegium Veterinarium

Person responsible for Biochemistry:

Department of Biochemistry, Faculty of Veterinary Medicine

Prof dr hab Marta Kankofer – Collegium Veterinarium, room no 218 (groundfloor)

Dates	Title of Class
22.02.2024	Aminoacid composition of protein chemical identification of aminoacids essential/nonessential aminoacids titration of aminoacids in accordance to Sörensen bioch_2_protocol_Aminoacid_composition_of _protein bioch_2_presentation_Aminoacid_composition_of _protein bioch_2_theory_Aminoacid_composition_of _protein Report from practicals
29.02.2024	Physico-chemical properties of proteins salting out of proteins the determination of isoelectric point precipitation of proteins with cations of heavy metals and acidic anions bioch_2_theory_physico_chem_properties bioch_2_presentation_physico_chem_properties bioch_2_protocol_physico_chem_properties.docx Report from practicals
07.03.2024	Quantitative methods for protein determinations the preparation of standard curve for biuret method quantitative determination of protein in sample bioch_1_presentation_Quantitative_methods_for_protein bioch_1_protocol_Quantitative_methods_for_protein bioch_1_theory_Aminoacid_composition_of _protein Report from practicals
14.03.2024	AMINOACIDS, PEPTIDES, PROTEINS – structures, biological meaning Test_1_Aminoacids_Proteins.doc
21.03.2024	Enzymatic kinetics 1 general properties of enzymes – the influence of pH, activators, temperature on activity of enzymes based on salivary amylase bioch_1_Presentation_Enzymatic_kinetics 1_Amylase bioch_1_protocol_Enzymatic_kinetics 1_Amylase bioch_1_theory_Enzymatic_kinetics 1_Amylase Report from practicals
28.03.2024	Seminar – coenzymes
04.04.2024	Enzymatic kinetics 2 the influence of enzymes concentration and time on the velocity of saccharose hydrolysis by use of invertase bioch_1_presentation_Enzymatic_kinetics_2_invertase bioch_7_protocol_enzymes 2_invertase bioch_1_theory_Enzymatic_kinetics_2_invertase Report from practicals
11.04.2024	Enzymatic kinetics 3 the influence of substrate concentration on the velocity of saccharose hydrolysis by use of invertase Michaelis constant bioch_1_Presentation_Enzymatic_kinetics_3_Michaelis bioch_1_Protocol_Enzymatic_kinetics_3_Michaelis bioch_1_theory_Enzymatic_kinetics_3_Michaelis Report from practicals
18.04.2024	ENZYMES, COENZYMES – structures, biological meaning Test_2_Enzymes_and_coenzymes.doc
25.04.2024	Nucleic acids preparation and isolation of RNA hydrolysis and qualitative analysis of RNA bioch_1_additional_materials bioch_1_presentation_Nucleic_acids bioch_1_protocol_Nucleic_acids Report from practicals
02.05.2024	Seminar – biosynthesis of proteins
09.05.2024	Methods for purification and separation of biological material – seminar
16.05.2024	Protein separation methods (sieve chromatography by use of Sephadex®) bioch_1_presentation_chromatography bioch_1_protocol_chromatography bioch_1_theory_chromatographyReport from practicals
23.05.2024	NUCLEIC ACIDS, BIOSYNTHESIS OF PROTEIN – structures, biological meaning Test_3_Nucleic
06.06.2024	Completion of practicals – evaluation of practical skills

Supplementary materials

Item	Files
Lectures	Proteins proteins_1 proteins_2 Enzymes enzymes-konspekt Coenzymes coenzymes Nucleic acids nucleotides https://up.lublin.pl/weterynaria/wp-content/uploads/sites/3/2022/03/nucleotides-1-konspekt.pdf Protein synthesis protein synthesis-konspekt
Conspectuses	Digestion Oxidative stress
Books	Koolman J, Roehm KH – Color atlas of biochemistry Kaneko et al. – Clinical biochemistry of domestic animals Salway JE – Medical biochemistry at a glance Harper`s biochemistry Stryer – Biochemistry Ron Fry – How to study?

University of Life Sciences in Lublin

13 Akademicka Street, 20-950 Lublin

VATIN 712 010 37 75

REGON no. 000001896

ePUAP: /UP-Lublin/SkrytkaESP

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Faculty of Veterinary Medicine

13 Akademicka Street

20-950 Lublin

Dean’s Office: phone no. (+48) 81 528 47 24,
(+48) 81 528 47 22

PLIKI COOKIES

MAPA STRONY

2020