Biochemistry course covers 2 semester and 150 didactic hours.

Frontal lectures (30 h per semester) are in weekly intervals during 15 consecutive weeks 2 h each and cover selected topics necessary to understand metabolism of aminoacids, nucleic acids, sugars and lipids. Moreover, the control and regulation of metabolic pathways is discussed.

Practical classes (45 h per semester) are obligatory and are in weekly intervals during 15 consecutive weeks 3 h each. Students carry on chemical experiments by their own. Students should be prepared for coming class.

Detailed rules of laboratory classes and work safety are available on web page and will be described during the first class. White coat is required for practical classes.

Students get access to digital platform with virtual cases for self-study.

Detailed description of topics is available in Syllabuses.

Lectures are held in Lecture hall A – Collegium Veterinarium

Practicals are held in laboratory no 120 – Collegium Veterinarium

Person responsible for Biochemistry:

Department of Biochemistry, Faculty of Veterinary Medicine

Prof dr hab Marta Kankofer – Collegium Veterinarium, room no 218 (groundfloor)

No. Dates Title of Class
1.  27.02.2025 Aminoacid composition of protein

  • chemical identification of aminoacids
  • essential/nonessential aminoacids
  • titration of aminoacids in accordance to Sörensen

Download: 
https://up.lublin.pl/weterynaria/wp-content/uploads/sites/3/2022/03/bioch_2_presentation_Aminoacid_composition_of-_protein.pdf       https://up.lublin.pl/weterynaria/wp-content/uploads/sites/3/2022/03/bioch_2_theory_Aminoacid_composition_of-_protein.pdf https://up.lublin.pl/weterynaria/wp-content/uploads/sites/3/2022/03/bioch_2_protocol_Aminoacid_composition_of-_protein.pdf
Report from practicals

2.  06.03.2025 Physico-chemical properties of proteins 

  • salting out of proteins
  • the determination of isoelectric point
  • precipitation of proteins with cations of heavy metals and acidic anions

Download:
https://up.lublin.pl/weterynaria/wp-content/uploads/sites/3/2022/03/bioch_2_presentation_physico_chem_properties-1.pdf https://up.lublin.pl/weterynaria/wp-content/uploads/sites/3/2022/03/bioch_2_theory_physico_chem_properties.pdf https://up.lublin.pl/weterynaria/wp-content/uploads/sites/3/2022/03/bioch_2_protocol_physico_chem_properties.docx-1.pdf
Report from practicals

3. 13.03.2025 Quantitative methods for protein determinations

  • the preparation of standard curve for biuret method
  • quantitative determination of protein in sample

Download:
https://up.lublin.pl/weterynaria/wp-content/uploads/sites/3/2022/03/bioch_1_presentation_Quantitative_methods_for_protein.pdf https://up.lublin.pl/weterynaria/wp-content/uploads/sites/3/2022/03/bioch_1_theory_Aminoacid_composition_of-_protein.pdf  https://up.lublin.pl/weterynaria/wp-content/uploads/sites/3/2022/03/bioch_1_protocol_Quantitative_methods_for_protein.pdf  
Report from practicals

4. 20.03.2025 AMINOACIDS, PEPTIDES, PROTEINS – structures, biological meaning
Download:
Test_1_Aminoacids_Proteins.doc
5. 27.03.2025 Enzymatic kinetics 1

  • general properties of enzymes – the influence of pH, activators, temperature on activity of enzymes based on salivary amylase

Download:
Enzymes_1_amylase_pH_temp_MJ https://up.lublin.pl/weterynaria/wp-content/uploads/sites/3/2022/03/bioch_1_theory_Enzymatic_kinetics-1_Amylase.pdf https://up.lublin.pl/weterynaria/wp-content/uploads/sites/3/2022/03/bioch_1_protocol_Enzymatic_kinetics-1_Amylase.pdf
Report from practicals

6. 10.04.2025 Enzymatic kinetics 2

  • the influence of enzymes concentration and time on the velocity of saccharose hydrolysis by use of invertase

Download:
https://up.lublin.pl/weterynaria/wp-content/uploads/sites/3/2022/03/bioch_1_presentation_Enzymatic_kinetics_2_invertase.pdf https://up.lublin.pl/weterynaria/wp-content/uploads/sites/3/2022/03/bioch_1_theory_Enzymatic_kinetics_2_invertase.pdf https://up.lublin.pl/weterynaria/wp-content/uploads/sites/3/2024/04/bioch_7_protocol_enzymes-2_.pdf
Report from practicals

7. 17.04.2025 Enzymatic kinetics 3

  • the influence of substrate concentration on the velocity of saccharose hydrolysis by use of invertase
  • Michaelis constant

Download:
https://up.lublin.pl/weterynaria/wp-content/uploads/sites/3/2022/03/bioch_1_Presentation_Enzymatic_kinetics_3_Michaelis.pdf https://up.lublin.pl/weterynaria/wp-content/uploads/sites/3/2022/03/bioch_1_theory_Enzymatic_kinetics_3_Michaelis.pdf https://up.lublin.pl/weterynaria/wp-content/uploads/sites/3/2022/03/bioch_1_Protocol_Enzymatic_kinetics_3_Michaelis.pdf
Report from practicals

8. 24.04.2025 ENZYMES, COENZYMES – structures, biological meaning
Download:
Test_2_Enzymes_and_coenzymes.doc
9. 08.05.2025 Nucleic acids

  • preparation and isolation of RNA
  • hydrolysis and qualitative analysis of RNA

Download:
https://up.lublin.pl/weterynaria/wp-content/uploads/sites/3/2022/03/bioch_1_presentation_Nucleic_acids.pdf https://up.lublin.pl/weterynaria/wp-content/uploads/sites/3/2022/03/bioch_1_additional_materials.pdf https://up.lublin.pl/weterynaria/wp-content/uploads/sites/3/2024/04/bioch_1_protocol_Nucleic_acids.pdf
Report from practicals

10. 15.05.2025 Seminar – methods for purification and separation of biological material 
11. 22.05.2025 Protein separation methods (sieve chromatography by use of  Sephadex®)
Download:
bioch_1_presentation_chromatography bioch_1_protocol_chromatography bioch_1_theory_chromatographyReport from practicals
12. 29.05.2025 NUCLEIC ACIDS, BIOSYNTHESIS OF PROTEIN – structures, biological meaning
Download:
Test_3_Nucleic
13. 05.06.2025 Completion of practicals – evaluation of practical skills
14. 12.06.2025 Seminar – biosynthesis of proteins

 

Supplementary materials

Item Files
Lectures Proteins 
https://up.lublin.pl/weterynaria/wp-content/uploads/sites/3/2025/02/Proteins_1.pdf Proteins_2025

Enzymes 

Enzymes_lecture_2025

Coenzymes

coenzymes

Nucleic acids
nucleotides https://up.lublin.pl/weterynaria/wp-content/uploads/sites/3/2022/03/nucleotides-1-konspekt.pdf

Protein synthesis

protein synthesis-konspekt
Conspectuses Digestion
Oxidative stress
Books

Koolman J, Roehm KH – Color atlas of biochemistry

Kaneko et al. – Clinical biochemistry of domestic animals

Salway JE – Medical biochemistry at a glance

Harper`s biochemistry

Stryer – Biochemistry

Ron Fry – How to study?