Biochemistry II

Biochemistry course covers 2 semester and 150 didactic hours.
Frontal lectures (30 h per semester) are in weekly intervals during 15 consecutive weeks 2 h each and cover selected topics necessary to understand metabolism of aminoacids, nucleic acids, sugars and lipids. Moreover, the control and regulation of metabolic pathways is discussed.

Practical classes (45 h per semester) are obligatory and are in weekly intervals during 15 consecutive weeks 3 h each. Students carry on chemical experiments by their own. Students should be prepared for coming class.
Detailed rules of laboratory classes and work safety are available on web page and will be described during the first class. White coat is required for practical classes.

Students get access to digital platform with virtual cases for self-study.

Detailed description of topics is available in Syllabuses.

Lectures are held in Lecture hall A – Collegium Veterinarium
Practicals are held in laboratory no 120 – Collegium Veterinarium

Person responsible for Biochemistry:
Department of Biochemistry, Faculty of Veterinary Medicine
Prof dr hab Marta Kankofer – Collegium Veterinarium, room no 218 (groundfloor)

No.	Date	Title of Class
1.	3-10-203	bioch_2_report_hydrolases_peptidases bioch_2_Protocol_Hydrolases_peptidases_pepsin_trypsin bioch_2_Presentation_Hydrolases_peptidases_pepsin_trypsin
2	10-10-2023	Transferases – transaminases (alanine aminotransferase and asparagine aminotransferase) bioch_2_Protocol_Transferases_transaminases_alanine _aminotransferase_and_asparagine_aminotransferase (2) bioch_2_Presentation_Transferases_transaminases_alanine _aminotransferase_and_asparagine_aminotransferase bioch_2_report_transferases_transaminases_alanine _aminotransferase_and_asparagine_aminotransferase
3	17-10-2023	Evaluation of knowledge – ANABOLISM AND CATABOLISM OF AMINOACIDS AND NUCLEIC ACIDS bioch_2_Evaluation_1_ANABOLISM_AND _CATABOLISM_OF_AMINOACIDS_AND_NUCLEIC_ACIDS
4	24-10-2023	Hydrolases – carboxylases (pancreatic amylase and lipase) bioch_2_report_hydrolases_peptidases_pepsin_trypsin bioch_2_Protocol_Hydrolases_carboxylases_pancreatic_amylase_and_lipase bioch_2_Presentation_Hydrolases_carboxylases_pancreatic_amylase_and_lipase
5	31-10-2023	Oxidoreductases – dehydrogenases (succinate dehydrogenase) bioch_2_report_oxidoreductases_dehydrogenases_(succinate_dehydrogenase) bioch_2_protocol_Oxidoreductases_dehydrogenases_(succinate_dehydrogenase) bioch_2_presentation_Oxidoreductases_dehydrogenases_(succinate_dehydrogenase)
6	7-11-2023	Evaluation of knowledge – ANABOLISM AND CATABOLISM OF POLYSACCHARIDES bioch_2_Evaluation_2_ANABOLISM_AND_CATABOLISM_OF_POLYSACCHARIDES
7	14-11-2023	Hydrolases – acidic and basic phosphatase Theoretical report from practicals-Hydrolases_acidic_and_basic_phosphatase Characteristics of isoenzymes of phosphatases and their localisation Diagnostic meaning of phosphatase determinations The interpretation of obtained result bioch_2_Protocol_Hydrolases_acidic_and_basic_phosphatase bioch_2_Presentation_Hydrolases_acidic_and_basic_phosphatase
8	21-11-2023	Seminar – hormones bioch_2_report_seminary–hormones bioch_2_Seminar_hormones
9	28-11-2023	Evaluation of knowledge – ANABOLISM AND CATABOLISM OF LIPIDSbioch_2_Evaluation_3_ANABOLISM_AND_CATABOLISM_OF_LIPIDS
10	05-12-2023	Body fluids – blood Theoretical report from practicals- blood Meaning of particular plasma compounds Mechanism of action of protein buffer Haemolysis and its influence to laboratory results The interpretation of buffer properties of plasma The results of glucose and ion (Cl^–, SO₄^2-, HPO₄^2-, Ca²⁺, Fe³⁺) detection in plasma samples Red blood cells in hypertonic, hypotonic and isotonic solutions – interpretation. bioch_2_Presentation_Blood_fluids bioch_2_Protocol_Blood_fluids
11	12-12-2023	Body fluids – physiological and pathological urine Theoretical report from practicals- urine Physical examination of urine – how it is done and what info provides about the patient What is the source of ketone bodies in urine? How to interpret the presence of protein in urine? How protein content in urine can be determined in field conditions? Why in selected determinations in urine deproteination is done? explain bioch_2_Protocol_Body_fluids_Physiological_and_pathological_urine Presentation_Body_fluids_Physiological_and_patholog
12	19-12-2023	Body fluids – milk, bile Theoretical report from practicals- milk,bile How milk is produced? what is the first step? Which compounds are of mammary gland origin and which reactions are necessary Do the content of milk differs between females? why? Why colostrum proteins are not digested during first hours of newborn life How hiperimmunised colostrum is produced and how can be used? Why “phenolophtalein milk” is decoloured under the influence of lipase? Which compounds of bile are responsible for its emulsification properties – explain Describe secretive and excretive role of bile What is the background of emulsifying properties of bile – explain based on last experiments bioch_2_PresentationBody_fluids _milk,_bile bioch_2_Protocol_Body_fluids _milk,_bile
13	9-01-2024	Evaluation of knowledge – BIOCHEMISTRY OF CHOSEN ORGANS AN TISSUES. BODY FLUIDS, HORMONES BIOCHEMISTRY_OF_CHOSEN_ORGANS_AND_TISSUES_BOD_FLUIDS_HORMONES
14	16-01-2024	Evaluation of practical skills
15.	23-01-2024	Making up for missed exercises