Biochemistry course covers 2 semester and 150 didactic hours.

Frontal lectures (30 h per semester) are in weekly intervals during 15 consecutive weeks 2 h each and cover selected topics necessary to understand metabolism of aminoacids, nucleic acids, sugars and lipids. Moreover, the control and regulation of metabolic pathways is discussed.

Practical classes (45 h per semester) are obligatory and are in weekly intervals during 15 consecutive weeks 3 h each. Students carry on chemical experiments by their own. Students should be prepared for coming class.

Detailed rules of laboratory classes and work safety are available on web page and will be described during the first class. White coat is required for practical classes.

Students get access to digital platform with virtual cases for self-study.

Detailed description of topics is available in Syllabuses.

Lectures are held in Lecture hall A – Collegium Veterinarium

Practicals are held in laboratory no 120 – Collegium Veterinarium

Person responsible for Biochemistry:

Department of Biochemistry, Faculty of Veterinary Medicine

Prof dr hab Marta Kankofer – Collegium Veterinarium, room no 218 (groundfloor)

Dates Title of Class

Aminoacid composition of protein

  • chemical identification of aminoacids
  • essential/nonessential aminoacids
  • titration of aminoacids in accordance to Sörensen

bioch_2_protocol_Aminoacid_composition_of _protein bioch_2_presentation_Aminoacid_composition_of _protein bioch_2_theory_Aminoacid_composition_of _protein

Report from practicals


Physico-chemical properties of proteins 

  • salting out of proteins
  • the determination of isoelectric point
  • precipitation of proteins with cations of heavy metals and acidic anions

bioch_2_theory_physico_chem_properties bioch_2_presentation_physico_chem_properties bioch_2_protocol_physico_chem_properties.docx

Report from practicals


Quantitative methods for protein determinations

  • the preparation of standard curve for biuret method
  • quantitative determination of protein in sample

bioch_1_presentation_Quantitative_methods_for_protein bioch_1_protocol_Quantitative_methods_for_protein bioch_1_theory_Aminoacid_composition_of _protein 

Report from practicals 

14.03.2024 AMINOACIDS, PEPTIDES, PROTEINS – structures, biological meaning

Enzymatic kinetics 1

  • general properties of enzymes – the influence of pH, activators, temperature on activity of enzymes based on salivary amylase

bioch_1_Presentation_Enzymatic_kinetics 1_Amylase bioch_1_protocol_Enzymatic_kinetics 1_Amylase bioch_1_theory_Enzymatic_kinetics 1_Amylase 

Report from practicals

28.03.2024 Seminar – coenzymes

Enzymatic kinetics 2

  • the influence of enzymes concentration and time on the velocity of saccharose hydrolysis by use of invertase

bioch_1_presentation_Enzymatic_kinetics_2_invertase bioch_7_protocol_enzymes 2_invertase bioch_1_theory_Enzymatic_kinetics_2_invertase

Report from practicals


Enzymatic kinetics 3

  • the influence of substrate concentration on the velocity of saccharose hydrolysis by use of invertase
  • Michaelis constant

bioch_1_Presentation_Enzymatic_kinetics_3_Michaelis bioch_1_Protocol_Enzymatic_kinetics_3_Michaelis bioch_1_theory_Enzymatic_kinetics_3_Michaelis

Report from practicals

18.04.2024 ENZYMES, COENZYMES – structures, biological meaning

Nucleic acids

  • preparation and isolation of RNA
  • hydrolysis and qualitative analysis of RNA

bioch_1_additional_materials bioch_1_presentation_Nucleic_acids bioch_1_protocol_Nucleic_acids

02.05.2024 Seminar – biosynthesis of proteins
09.05.2024 Methods for purification and separation of biological material – seminar

Protein separation methods (sieve chromatography by use of  Sephadex®)

bioch_1_presentation_chromatography bioch_1_protocol_chromatography bioch_1_theory_chromatography

23.05.2024 NUCLEIC ACIDS, BIOSYNTHESIS OF PROTEIN – structures, biological meaning
06.06.2024 Completion of practicals – evaluation of practical skills

Supplementary materials

Item Files

Proteins proteins_1 proteins_2

Enzymes enzymes-konspekt

Coenzymes coenzymes

Nucleotides-1 nucleotides

Nucleotides-2 nucleotides-1-konspekt protein synthesis-konspekt


Koolman J, Roehm KH – Color atlas of biochemistry

Kaneko et al. – Clinical biochemistry of domestic animals

Salway JE – Medical biochemistry at a glance

Harper`s biochemistry

Stryer – Biochemistry

Ron Fry – How to study?