Agata Hahaj-Siembida
III rok Szkoły Doktorskiej
Dyscyplina: Weterynaria

From February 11, 2024 to March 3, 2024 I completed a research internship at Charles University (Univerzita Karlova) in Prague, First Faculty of Medicine, Institute of Pathological Physiology. The main aim of the trip was the analysis of miRNA in sheep plasma in connection with inflammation (with increased levels of cytokines) during pregnancy.

During the internship, I expanded my knowledge of molecular techniques, especially the RT-PCR method. The research was based mainly on the extraction of total RNA from sheep blood, selection of target microRNA/mRNA and reverse transcription PCR (RT-PCR).

In addition to working in the laboratory, I had the opportunity to visit the beautiful capital of the Czech Republic. Prague is a very charming city located on the Wełtawa River. During my free time, I had the opportunity to walk across the Charles Bridge, visit the beautiful Prague Castle and the Cathedral of St. Vitus, as well as the Golden Lane and the Astronomical Clock. I also had the opportunity to visit the Prague Zoo.

The internship at Charles University in Prague enabled me to improve my skills in planning experiments and expand my knowledge of molecular techniques. Moreover, I had the opportunity to meet many interesting people and work in an international team. Thanks to this, I gained valuable experience and international connections.