During the period from 11^th February  to 11^th March, 2024, third-year doctoral student Msc Maciej Bryś completed a research internship at the Department of Biochemistry at the Faculty of Life Sciences of Palacky University in Olomouc (Czech Republic). The main purpose of the trip was to familiarize himself with biochemical methods for assessing the physiology of honey bees in terms of immunity.

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Poland and the Czech Republic are affected by problems related to the mechanization of agriculture, the use of plant protection products, climate change, and the loss of plant biodiversity, which directly and indirectly impact pollinating insects, including honey bees.

Professor Jiří Danihlík’s team focuses on studying the individual immunity of honey bees. This topic aligns with my research task carried out as part of the Individual Research Plan at the Doctoral School.

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The objectives of the internship included:

• gaining practical experience in biochemical analysis of honey bee immunity peptides
• engaging in discussions and exchanging scientific views
• establishing scientific connections
• developing soft skills
• overcoming language barriers in English
• learning about international culture and customs

During the internship, I learned immunochemical methods (ELISA) used for detecting honey bee immune proteins.

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I conducted analyses of bee immune proteins using liquid chromatography combined with mass spectrometry. Additionally, I familiarized myself with other methods used in bee immunity research at Palacky University.

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Furthermore, I acquainted myself with other methods used in bee immunity research at Palacky University.

In addition to the scientific aspects, this internship allowed me to discover remarkable cultural and architectural attractions of the Czech Republic.

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In summary, the internship at the Department of Biochemistry provided an opportunity to learn modern techniques for studying insect immune proteins. I acquired practical skills in handling specialized tools for measuring biological compounds in bee tissues. The memories will last long in my mind, and the scientific contacts gained will be maintained.