{"id":453233,"date":"2025-01-17T09:57:52","date_gmt":"2025-01-17T08:57:52","guid":{"rendered":"https:\/\/up.lublin.pl\/blog\/agata-hahaj-siembida-msc-charles-university-in-prague-czech-republic\/"},"modified":"2025-03-28T08:18:29","modified_gmt":"2025-03-28T07:18:29","slug":"agata-hahaj-siembida-msc-charles-university-in-prague-czech-republic","status":"publish","type":"post","link":"https:\/\/up.lublin.pl\/en\/blog\/agata-hahaj-siembida-msc-charles-university-in-prague-czech-republic\/","title":{"rendered":"Agata Hahaj-Siembida, MSc, Charles University in Prague, Czech Republic"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"453233\" class=\"elementor elementor-453233 elementor-453203\" data-elementor-settings=\"[]\">\n\t\t\t\t\t\t<div class=\"elementor-inner\">\n\t\t\t\t\t\t\t<div class=\"elementor-section-wrap\">\n\t\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-3d0c470 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"3d0c470\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t\t\t<div class=\"elementor-row\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-062edb5\" data-id=\"062edb5\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-4e70fc0 elementor-widget elementor-widget-m5c\" data-id=\"4e70fc0\" data-element_type=\"widget\" data-widget_type=\"m5c.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<section class=\"mn-m5-c mn-m5-d2 mn-bg-white\">\n\t<div class=\"mn-grid\">\n\t\t\t\t<div class=\"mn-content mn-grid-9\">\n\t\t\t                <div class=\"mn-content-block active\">\n\t                                                        <div class=\"block-text\">\n\t                                        <p><a href=\"https:\/\/up.lublin.pl\/wp-content\/uploads\/2025\/03\/logo-ster-ENG.png\"><img loading=\"lazy\" class=\"aligncenter size-full wp-image-466827\" src=\"https:\/\/up.lublin.pl\/wp-content\/uploads\/2025\/03\/logo-ster-ENG.png\" alt=\"logotypes of Polish National Agency for Academic Exchange, ster programme and Doctoral School of the University of Life Sciences in Lublin\" width=\"720\" height=\"92\" \/><\/a><\/p>\n<p><strong>Agata Hahaj-Siembida <br \/><\/strong><strong>4th Year of Doctoral School<br \/><\/strong><strong>Discipline: Veterinary Medicine <\/strong><\/p>\n<p>From November 6 to December 6, 2024, I&nbsp;completed a&nbsp;research internship at Charles University in Prague at the Laboratory of Molecular Mechanisms of Blood Diseases (MMBD), Institute of Pathological Physiology, First Faculty of Medicine. My research supervisor was RNDr. Karina Savvulidi Vargova, Ph.D. The main goal of the internship was to analyze miRNA in the plasma of sheep in connection with inflammation (with increased cytokine levels) during pregnancy.<\/p>\n<p><a href=\"https:\/\/up.lublin.pl\/wp-content\/uploads\/2025\/01\/staz_ahs_2.jpg\"><img loading=\"lazy\" class=\"aligncenter size-full wp-image-453222\" src=\"https:\/\/up.lublin.pl\/wp-content\/uploads\/2025\/01\/staz_ahs_2.jpg\" alt=\"\" width=\"1015\" height=\"756\" \/><\/a><\/p>\n<p>During the internship, I&nbsp;expanded my knowledge of molecular techniques, especially the qPCR method. The research was conducted mainly based on RNA isolation from the blood of BCP sheep, selection of target microRNA\/mRNA and reverse transcription polymerase chain reaction (RT-PCR), as well as real-time polymerase chain reaction (qPCR).<\/p>\n<p>In addition to working in the laboratory, I&nbsp;had the opportunity to attend a&nbsp;lecture on miRNA given by Petr Svoboda. During my free time I&nbsp;had the opportunity to visit beautiful Prague situated on the Vltava River. I&nbsp;had the opportunity to walk across Charles Bridge, visit the beautiful Prague Castle with St. Vitus Cathedral, St. Ludmila&#8217;s Church and the Polish parish in Prague as well as the Prague astronomical clock. I&nbsp;also had the opportunity to visit the Prague Christmas market.<\/p>\n<p>The internship at Charles University in Prague allowed me to expand my practical skills in laboratory work. In addition, I&nbsp;had the opportunity to meet many interesting people and work in an international team. Thanks to this, I&nbsp;gained valuable experience and international contacts.<\/p>\n<p><em>This foreign mobility has been supported by the Polish National Agency for Academic Exchange (NAWA) under the STER programme: \u201eActions towards the internationalization of the Doctoral School of the University of Life Sciences in Lublin (I-SDUPL)\u201d.<\/em><\/p>\n                                        <\/div>\n\t\t\t\t\t\t                                                        <div class=\"mn-cards-list\">\n                                            <div class=\"mn-card-container\">\n                                                <div>\n                                                    <img src=\"https:\/\/up.lublin.pl\/wp-content\/themes\/up-lublin\/assets\/images\/doc-ico.svg\" alt=\"\" \/>\n                                                    <h5>visit report (.pdf)<\/h5>\n                                                <\/div>\n                                                <a href=\"https:\/\/up.lublin.pl\/wp-content\/uploads\/2025\/03\/Praga-list-grudzien-2024-\u2014-pdf-z-logo-i-info-1.pdf\" class=\"mn-more\" target=\"_blank\">Read more<\/a>\n                                            <\/div>\n                                        <\/div>\n\t\t\t\t\t\t                                <\/div>\n\t\t\t\t                <div class=\"mn-content-block\">\n\t                                <\/div>\n\t\t\t\t                <div class=\"mn-content-block\">\n\t                                <\/div>\n\t\t\t\t                <div class=\"mn-content-block\">\n\t                                <\/div>\n\t\t\t\t                <div class=\"mn-content-block\">\n\t                                <\/div>\n\t\t\t\t                <div class=\"mn-content-block\">\n\t                                <\/div>\n\t\t\t\t                <div class=\"mn-content-block\">\n\t                                <\/div>\n\t\t\t\t                <div class=\"mn-content-block\">\n\t                                <\/div>\n\t\t\t\t                <div class=\"mn-content-block\">\n\t                                <\/div>\n\t\t\t\t                <div class=\"mn-content-block\">\n\t                                <\/div>\n\t\t\t\t                <div class=\"mn-content-block\">\n\t                                <\/div>\n\t\t\t\t                <div class=\"mn-content-block\">\n\t                                <\/div>\n\t\t\t\t                <div class=\"mn-content-block\">\n\t                                <\/div>\n\t\t\t\t                <div class=\"mn-content-block\">\n\t                                <\/div>\n\t\t\t\t                <div class=\"mn-content-block\">\n\t                                <\/div>\n\t\t\t\t                <div class=\"mn-content-block\">\n\t                                <\/div>\n\t\t\t\t                <div class=\"mn-content-block\">\n\t                                <\/div>\n\t\t\t\t                <div class=\"mn-content-block\">\n\t                                <\/div>\n\t\t\t\t                <div class=\"mn-content-block\">\n\t                                <\/div>\n\t\t\t\t                <div class=\"mn-content-block\">\n\t                                <\/div>\n\t\t\t\t\t\t<\/div>\n\t<\/div>\n<\/section>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Agata Hahaj-Siembida 4th Year of Doctoral SchoolDiscipline: Veterinary Medicine From November 6 to December 6, 2024, I&nbsp;completed a&nbsp;research internship at Charles University in Prague at the Laboratory of Molecular Mechanisms of Blood Diseases (MMBD), Institute of Pathological Physiology, First Faculty of Medicine. My research supervisor was RNDr. Karina Savvulidi Vargova, Ph.D. The main goal of [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":453210,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"elementor_header_footer","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0},"categories":[5545,5721],"tags":[],"_links":{"self":[{"href":"https:\/\/up.lublin.pl\/en\/wp-json\/wp\/v2\/posts\/453233"}],"collection":[{"href":"https:\/\/up.lublin.pl\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/up.lublin.pl\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/up.lublin.pl\/en\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/up.lublin.pl\/en\/wp-json\/wp\/v2\/comments?post=453233"}],"version-history":[{"count":16,"href":"https:\/\/up.lublin.pl\/en\/wp-json\/wp\/v2\/posts\/453233\/revisions"}],"predecessor-version":[{"id":467770,"href":"https:\/\/up.lublin.pl\/en\/wp-json\/wp\/v2\/posts\/453233\/revisions\/467770"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/up.lublin.pl\/en\/wp-json\/wp\/v2\/media\/453210"}],"wp:attachment":[{"href":"https:\/\/up.lublin.pl\/en\/wp-json\/wp\/v2\/media?parent=453233"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/up.lublin.pl\/en\/wp-json\/wp\/v2\/categories?post=453233"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/up.lublin.pl\/en\/wp-json\/wp\/v2\/tags?post=453233"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}